Yes, several players from Hamburger SV (HSV) have been part of FIFA World Cup-winning national teams.
One notable player is Uwe Seeler, who played for HSV for his entire career and was part of the West Germany national team that won the 1954 World Cup. He was also a member of the West Germany squad that finished as runners-up in the 1966 World Cup.
Another player is Felix Magath, who played for HSV in the 1970s and was part of the West Germany team that won the 1974 World Cup. Magath played in two matches during the tournament and was a key member of the team.
In addition, Thomas von Heesen, who played for HSV in the 1980s, was part of the West Germany squad that won the 1990 World Cup in Italy.
More recently, Marcell Jansen, who played for HSV from 2008 to 2015, was part of the Germany squad that won the 2014 World Cup in Brazil.